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reaction system includes primescript ii rtase  (TaKaRa)


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    Structured Review

    TaKaRa reaction system includes primescript ii rtase
    Reaction System Includes Primescript Ii Rtase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+reaction/PrimeScript+II+Reverse+Transcriptase/pm41922812-41-1-9
    Average 96 stars, based on 734 article reviews
    reaction system includes primescript ii rtase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Suppression of Sebum Production by Vemurafenib Through Paradoxical ERK Activation Resulted in the Inhibition of the mTOR Pathway in 5α‐Dihydrotestosterone‐Differentiated Hamster Sebocytes In Vitro
    Article Snippet: .. Total RNA was isolated from the cells using ISOGEN II (Nippon Gene, Toyama, Japan), and then an aliquot of RNA (500 ng) was subjected to a reverse transcriptase reaction for the synthesis of cDNA using a PrimeScript RT reagent (Takara Bio, Shiga, Japan) according to the manufacturer's instructions. .. Aliquots (an equivalent of 2.5 ng of total RNA) of the transcript were subjected to real‐time PCR for SCD‐1, DGAT‐1, PLIN‐1, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) mRNAs using THUNDERBRID SYBR qPCR Mix (Toyobo, Osaka, Japan) and their specific primers (Table ).

    Reverse Transcription:

    Article Title: Suppression of Sebum Production by Vemurafenib Through Paradoxical ERK Activation Resulted in the Inhibition of the mTOR Pathway in 5α‐Dihydrotestosterone‐Differentiated Hamster Sebocytes In Vitro
    Article Snippet: .. Total RNA was isolated from the cells using ISOGEN II (Nippon Gene, Toyama, Japan), and then an aliquot of RNA (500 ng) was subjected to a reverse transcriptase reaction for the synthesis of cDNA using a PrimeScript RT reagent (Takara Bio, Shiga, Japan) according to the manufacturer's instructions. .. Aliquots (an equivalent of 2.5 ng of total RNA) of the transcript were subjected to real‐time PCR for SCD‐1, DGAT‐1, PLIN‐1, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) mRNAs using THUNDERBRID SYBR qPCR Mix (Toyobo, Osaka, Japan) and their specific primers (Table ).

    Article Title: Genetic and Antigenic Variability in VP4 and VP7 of Group A Human Rotavirus in Yunnan, China, from 2015 to 2020
    Article Snippet: The virus RNA was extracted by QIAamp ® Viral RNA Mini Kit (Qiagen, Hilden, Germany, Code No. 52904). .. Reverse transcriptase reaction was operated by PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara, Dalian, China, Code No. 6210A). ..

    Article Title: Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modeling
    Article Snippet: RNA was extracted from RAW264.7 and BMDMs using the universal RNA extraction reagent TRIzol (15596018, Invitrogen). .. Subsequently, a reverse transcriptase reaction was performed following the manufacturer’s protocol with the PrimeScriptTM FAST RT reagent kit with gDNA Eraser (RR092A, Takara). .. The resulting cDNA was then combined with TB Green Premix Ex TaqTM II (RR820A, Takara), as per the manufacturer’s instructions, along with 4 pmol of both forward and reverse primers to quantify the mRNA levels of mouse genes.

    Article Title: Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix.
    Article Snippet: Total RNA was extracted from skeletal muscle tissue using TRIzol reagent (Invitrogen). .. RNA was transcribed into cDNA via a standard reverse transcriptase reaction using the PrimeScriptTM first-strand cDNA Synthesis Kit (Takara, Shiga, Japan), following the manufacturer’s protocol. .. These cDNAs were analyzed with quantitative real-time PCR (qPCR). qPCR was performed on a 96- well CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratory, Tokyo, Japan) using a THUNDER-BIRD Probe qPCR Mix (TOYOBO, Osaka, Japan).

    Article Title: Transcriptional regulators SP110 and SP140 modulate inflammatory response genes in Mycobacterium tuberculosis -infected human macrophages
    Article Snippet: .. Total RNA was extracted using RNeasy Mini Kit (Qiagen), and reverse transcriptase reaction was performed using Prime Script Reverse Transcriptase (Takara). .. Subsequently, RT-qPCR was performed using TaqMan Universal Master Mix (Thermo Fisher Scientific). lists the primers and probes used in this study.

    Article Title: Genetic and Antigenic Variability in VP4 and VP7 of Group A Human Rotavirus in Yunnan, China from 2015-2020.
    Article Snippet: 2.3 Nucleic acid extraction and sequencing The virus RNA was extracted by QIAamp® Viral RNA Mini Kit ( Qiagen, Hilden, Germany, Code No. 52904 ). .. Reverse transcriptase reaction was operated by PrimeScriptTM II 1st Strand cDNA Synthesis Kit( Takara, Dalian, China, Code No. 6210A ). ..

    Article Title: Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix
    Article Snippet: Total RNA was extracted from skeletal muscle tissue using TRIzol reagent (Invitrogen). .. RNA was transcribed into cDNA via a standard reverse transcriptase reaction using the PrimeScript TM first-strand cDNA Synthesis Kit (Takara, Shiga, Japan), following the manufacturer’s protocol. .. These cDNAs were analyzed with quantitative real-time PCR (qPCR). qPCR was performed on a 96-well CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratory, Tokyo, Japan) using a THUNDER-BIRD Probe qPCR Mix (TOYOBO, Osaka, Japan).

    cDNA Synthesis:

    Article Title: Genetic and Antigenic Variability in VP4 and VP7 of Group A Human Rotavirus in Yunnan, China, from 2015 to 2020
    Article Snippet: The virus RNA was extracted by QIAamp ® Viral RNA Mini Kit (Qiagen, Hilden, Germany, Code No. 52904). .. Reverse transcriptase reaction was operated by PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara, Dalian, China, Code No. 6210A). ..

    Article Title: Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix.
    Article Snippet: Total RNA was extracted from skeletal muscle tissue using TRIzol reagent (Invitrogen). .. RNA was transcribed into cDNA via a standard reverse transcriptase reaction using the PrimeScriptTM first-strand cDNA Synthesis Kit (Takara, Shiga, Japan), following the manufacturer’s protocol. .. These cDNAs were analyzed with quantitative real-time PCR (qPCR). qPCR was performed on a 96- well CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratory, Tokyo, Japan) using a THUNDER-BIRD Probe qPCR Mix (TOYOBO, Osaka, Japan).

    Article Title: Genetic and Antigenic Variability in VP4 and VP7 of Group A Human Rotavirus in Yunnan, China from 2015-2020.
    Article Snippet: 2.3 Nucleic acid extraction and sequencing The virus RNA was extracted by QIAamp® Viral RNA Mini Kit ( Qiagen, Hilden, Germany, Code No. 52904 ). .. Reverse transcriptase reaction was operated by PrimeScriptTM II 1st Strand cDNA Synthesis Kit( Takara, Dalian, China, Code No. 6210A ). ..

    Article Title: Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix
    Article Snippet: Total RNA was extracted from skeletal muscle tissue using TRIzol reagent (Invitrogen). .. RNA was transcribed into cDNA via a standard reverse transcriptase reaction using the PrimeScript TM first-strand cDNA Synthesis Kit (Takara, Shiga, Japan), following the manufacturer’s protocol. .. These cDNAs were analyzed with quantitative real-time PCR (qPCR). qPCR was performed on a 96-well CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratory, Tokyo, Japan) using a THUNDER-BIRD Probe qPCR Mix (TOYOBO, Osaka, Japan).

    other:

    Article Title: Methods of sensitizing tumors to treatment with immune checkpoint inhibitors
    Article Snippet: To generate model RNA templates (GFP, and OVA) for in vitro transcription (IVT), plasmids were linearized and purified with Qiagen PCR MiniElute kit before linearized DNA was transcribed using mMessenger RNA IVT kit (Life technologies, Invitrogen).



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